بررسی اثر ضددرد استرس به تنهایی یا در حضور آگونیست و آنتاگونیست گیرنده‌های CCK در درد نوروپاتیک به روش صفحه داغ در موش سوری

Introduction: Recent studies have shown that cold water swimming stress (CWSS) produces analgesia. Although exact mechanism of the analgesia is until unclear, it may attributed to CCK receptors. On the other hand, the effectiveness of analgesic drugs for treatment of neuropathic pain is limited. In the present study, the effects of CCK receptor agonist caerulein and antagonist proglumide , both alone and together on the CWSS- induced analgesia was investigated and compared in normal mice and those whose sciatic nerve was ligated. Material & Methods: In this research, fifty groups of nine male albino mice were used. As a model of neuropathy, the unilateral nerve ligation was made by a surgical procedure on the right hind limb. Pain sensitivity was measured by the hot plate test. Results: Different CWSS regimes (0.5-3 min) induced time-dependent analgesia. Nerve ligation did not alter the CWSS induced analgesia. Caerulein (0.025, 0.05 and 0.1 mg/kg) induced analgesia in both nerve ligated and intact animals. Different doses of proglumide (20, 40 and 60 mg/kg) alone produced analgesic effect. The response of caerulein was not inhibited by proglumide. In the stressed mice, both drugs and the combination of them showed analgesia but there appeared to be no potentiation in CWSS- induced analgesia. Our results indicate no significant relationship between CCK receptors and the CWSS- induced analgesia. Conclusion: Nevertheless, CWSS as an alternative to drug treatment in neuropathic pain requires more studies.